Friday, 16 March 2018



Fishes could use herbal medicines like humans too

Asian herbs are popular in many parts of the world owing to ‘natural origin’, low cost, and zero side effects. A recent study has found that it benefits fishes too.

Tilapia fishes are cultured for food in many countries including China, the Philippines, Taiwan, Indonesia, Thailand, India, and Bangladesh. Significant numbers of fishes die in summer months due to infection from a bacterium Entercoccus faecalis in aquaculture farms leading to huge financial losses.

A group of scientists from Bangladesh found that feeding fish with extracts of garlic and clove prevents death due to Entercoccus faecalis infection. This bacterium was resistant to most available antibiotics and herbal medication could help prevent disease and death in infected fishes, according to the study published in the journal Scientific Reports.

“A large number of herbs are effective against bacterial diseases in humans. Therefore, we thought that screening medicinal plants against fish pathogen may result in useful information and we, fortunately, got it”, says Tofazzal Islam, Professor at the Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh, who led the study. 

Left to right: A healthy tilapia fish (1) and those infected with the bacteria Entercoccus faecalis show swollen abdomen and hemorrhages at the base of pelvic fins (2) and erosion in caudal fin (3).

They collected tilapia fishes that looked ill from several aquaculture farms in Ghazipur in Bangladesh. Fishes showed excess slime, protruding opaque eyes, and swollen abdomen. The pelvic region had blood clots and the tail fin seemed washed off. They swam erratic, were lethargic, and had a decreased appetite. Since the cause of infection was unknown, the fishes were brought to the lab where the disease-causing bacteria were isolated from the skin, tail, gut, eye and brain.

After culturing on an artificial medium under controlled conditions, the bacteria were identified to be Enterococcus faecalis. Subsequent tests revealed that they were resistant to commercial antibiotics amoxicillin, ampicillin, cefradine, cefuroxime, erythromycin and penicillin-G and mildly susceptible to nitrofurantoin, azithromycin, gentamycin, levofloxacin, and vancomycin.

When healthy tilapia fishes were infected with the bacterium, they died in less than 72 hours indicating that this bacterium was highly virulent. Before exposing them to the bacterium, some fishes were fed with herbal medicines and others were left untreated. Those that received the herbal medication showed an average survival rate of approximately 70 percent compared to null in the infected untreated group. Commercial antibiotic azithromycin exhibited a survival rate of about 75 percent, which was similar to that bestowed by herbal medicines.

They selected garlic and clove after screening 23 different herbs that included zinger, turmeric, onion, pomegranate, chilli, tamarind, guava, and Indian gooseberry. “Our results suggest that medicinal plant extracts could be used as a potential alternative to the synthetic antibiotics to control fish diseases caused by Enterococcus faecalis in aquaculture”, claim scientists in their paper. They emphasize that mechanism of action of the plant crude extracts in preventing and fish disease has to be elucidated.

“Bangladesh is ranked fourth in aquaculture in the world. We are working now for elucidating the mode of action of crude extracts and also to make a commercial formulation for the aquaculture industry. It would hugely benefit the aquaculture industry in Bangladesh and other Asian countries as natural herbs are safe and cost-effective”, exclaims Professor Islam. 

The team consisted of Muntasir Rahman, Md Mahbubur Rahman, Suzan Chandra Deb, Md Shahanoor Alam, Md Jahangir Alam, and Md Tofazzal Islam. This study was done at the Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, and Shahjalal University of Science and Technology, Sylhet in Bangladesh.

Reference: Scientific Reports | 7: 3747 | DOI:10.1038/s41598-017-03673-1

By Bhavya Khullar

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